The differential expression of the anti-oxidation related genes in Echinococcus granulosus by real-time PCR
10.3969/j.issn.1002-2694.2010.01.001
- VernacularTitle:定量PCR研究细粒棘球蚴抗氧化相关基因的差异表达
- Author:
Qiulian HOU
;
Fuchun ZHANG
;
Wenbao ZHANG
;
Wulamu MAMUTI
;
Zhuangzhi ZHNAG
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
oxidative stress;
suppression subtractive hybridization;
real-time PCR;
dfferentially expressed gene
- From:
Chinese Journal of Zoonoses
2010;(1):1-5
- CountryChina
- Language:Chinese
-
Abstract:
To isolate the specific genes in protoscoleses (PSC) of Echinococcus granulosus under oxidative stresses from the SSH library constructed in the previous study, the gene expression in PSC under oxidative stresses was studied by using real-time PCR. The previously amplified library was sequenced and analyzed in GenBank with Blast research. Sequence analysis indicated that all clones in the SSH library contained the coding sequences, of which some clones showed homology in the GenBank and others were unknown. Differential expression of 4 genes randomly selected and the TPx gene in this library were studied with real-time PCR. It was demonstrated that the gene expression of S88 and H32-1 in oxidative tissues was 2.0 and 2.3 times higher than the un-oxidative stresses respectively. The TPx gene was up-regulated when PSC was induced with H_2H_2 of more than 0.8 mmol/L. These results implies that the up-regulated expression of the above-mentioned genes may be related with the related functions of anti-oxidative process in PSC and they may be used as the candidate genes for the study of anti-oxidation of E.granulosus.
