Cytotoxic effect of Guillain-Barre syndrome-associated and diarrhea-associated Campylobacter jejuni
10.3969/j.issn.1002-2694.2010.01.003
- VernacularTitle:格林巴利综合征相关及单纯腹泻相关空肠弯曲菌细胞毒作用分析
- Author:
Fanliang MENG
;
Maojun ZHANG
;
Lihua HE
;
Jianzhong ZHANG
- Publication Type:Journal Article
- Keywords:
Campylobacter jejuni;
cytotoxic effect;
HeLa cell
- From:
Chinese Journal of Zoonoses
2010;(1):10-12
- CountryChina
- Language:Chinese
-
Abstract:
In order to assess the cytotoxin effect of Campylobacter jejuni (C.jejuni), the whole cell lysates from 9 strains of Guillan-Barre Syndrome (GBS)- associated C.jejuni and 4 strains of diarrhea-associated C. jejuni were co-cultivated with HeLa cells at the concentration of 0.001-5.00 μg/mL in vitro. The morphologic change of HeLa cells was observed under Olympus BX51 microscope after treatment with different concentration of bacteria lysate in the following 4 days. The morphological changes including swelling, irregularity and lysis of the affected cells over 50% was selected as the cut off for positive change and C.jejuni 81-176 and Helicobacter J99 strains were chosen as the positive and negative control. It was found that the minimum concentration to induce the positive changes in 10 strains(8 GBS associated and 2 strains from diarrhea patients)was 0.1μg/mL and 1 strain with the positive change at the minimum concentration of 1 μg/mL. There was only one GBS-associated strain causing no morphologic change on HeLa cells at the concentration of 5 μg/mL. It was evident that the cytotoxic effect of C.jejuni strains on HeLa cell was strain-specific, and there was no significant difference in the cytotoxic effect on HeLa cell between GBS-associated and diarrhea associated C.jejuni strains.
