Identification and susceptibility test of bacteria isolated from miniature pigs in China
10.3969/j.issn.1002-2694.2010.01.012
- VernacularTitle:中国小型猪细菌分离鉴定及药敏试验
- Author:
Zhengqin GAO
;
Qiang ZHANG
;
Zhengming HE
;
Bingfei YUE
;
Qiang YE
- Publication Type:Journal Article
- Keywords:
miniature pig;
bacteria isolation;
identification;
16S rRNA
- From:
Chinese Journal of Zoonoses
2010;(1):46-52
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the prevalence and the antibiotic resistance of bacteria isolated from 25 miniature pigs. 45 bacterial strains were isolated, which were identified by biochemical assays, amplification of 16S rRNA genes by PCR and sequence analysis, and were evaluated for resistance to 30 antibiotics. The identification results showed that these bacteria belonged to Campylobacter (Campylobacter jejuni), Helicobacterium (Helicobacter pylori), Klebsiella (Klebsiella pneumoniae), Escherichia (Escherichia coli, Escherichia fergusonii), Pseudomonas (Pseudomonas aeruginosa), Stenotrophomonas (Stenotrophomonas maltophilia), Staphylococcus (Staphylococcus aureus, Staphylococcus haemolyticus, Staphylococcus simulans), Streptococcus (Streptococcus pneumoniae, Streptococcus suis, Streptococcus vestibularis, Streptococcus mitis, Gemella measles, Aerococcus viridans) and Bacillus (Bacillus subtilis, Bacillus licheniformis, Bacillus alvei, Bacterium megaterium). These bacteria were all susceptible to aztreonam and cephalothin. However, the resistence to furazolidone was found. Microbial population carried by miniature pigs in China had characters of diversity. Results of this study provided scientifical accordance for the microorganism monitoring of miniature pigs in China.
