Effect of Toxoplasma gondii infection on the placental apoptosis-related protein of BALB/c mice during the second trimester of pregnancy
10.3969/j.issn.1002-2694.2010.01.014
- VernacularTitle:弓形虫感染对妊娠中期小鼠胎盘组织中细胞凋亡相关蛋白表达的影响
- Author:
Huaxiang RAO
;
Yuying HOU
;
Huiping YUE
;
Yanxia HE
;
Yunhe ZHAO
;
Junfeng YANG
;
Hongye CHANG
;
Hui LIU
;
Jie ZHANG
;
Liping HOU
- Publication Type:Journal Article
- Keywords:
Toxoplasma gondii;
immunohistochemistry;
placenta;
apoptosis-related protein
- From:
Chinese Journal of Zoonoses
2010;(1):57-61
- CountryChina
- Language:Chinese
-
Abstract:
To observe the influence of the placental apoptosis on the expression of Bax,Bcl-2, Fas, FasLand TNF-α during the second trimester of pregnancy, mice of experimental group were intraperitoneal injected with 100 purified Toxoplasma gondii tachyzoites added in 0.2mL of PBS, while those of the control group were injected with 0.2 mL of sterile PBS (0.01 mol/L, pH 7.4) in the 8-th day of pregnancy. During the 12, 14, 16 and 18-th days of pregnancy, 5 mice both in experimental and control group were randomly killed and the expression levels of the apoptosis-related proteins Bax, Bcl-2, Fas, FasL and TNF-α in the placental tissues were determined by means of immunohistochemical methods. It was showed that the apoptosis-related protein expressed both in villus and decidua of the placenta, most of which were expressed in syneytiotrophoblast (ST). The positive cells with expression of Bax, Fas, FasL and TNF-α increased along with the increase of the pregnant days in both the experimental group and the control group, and the positive cells with expression of Bcl-2 decreased along with the increase of the pregnant days. It was also demonstrated that the positive cells with expression of Bax, Fas, FasL and TNF-α of the experimental group showed a higher percentage of expression than that of the control group on the same pregnant days, but the positive cells with Bcl-2 expression of the experimental group were fewer than that of the control group. It is concluded that the expression of apoptosis-related protein Bax, Bcl-2, Fas, FasL and TNF-α in the placenta were altered when the pregnant mice were infected with Toxoplasma gondii during the second trimester, which may induce the apoptosis through the endogenic and ectogenic pathway.
