Toxicity detection of heat-labile enterotoxin in non-toxic mutant of Escherichia coli and investigation on its adjuvant effect of E.coli heat-labile enterotoxin
10.3969/j.issn.1002-2694.2010.01.017
- VernacularTitle:大肠杆菌不耐热肠毒素无毒突变体mLT63毒性检测及佐剂效果研究
- Author:
Xuefei BAI
;
Jingyu GUO
;
Wanjun LEI
;
Guangcai DUAN
- Publication Type:Journal Article
- Keywords:
Escherichia coli heat labile enterotoxin;
mutants;
adjuvant
- From:
Chinese Journal of Zoonoses
2010;(1):69-71
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, the expression, purification, toxicity detection and the adjuvant effect of the heat-labile enterotoxin in non-toxic mutant mLT63 of Escherichia coli were investigated, in which the inductive expression was performed under optimal condition for inductive expression and the toxicity of the products obtained from inductive expression were tested for toxicity after being purified and concentrated with affinity chromatography. BALB/c mice were immunized orally with the mutant mLT63 associated with Helicobacter pylori (Hp) subunit vaccine UreB, Omp11. After immunization, the specific antibody levels in serum, extract from gastric tissues and fecal extract were determind by means of ELISA assay and the results were subjected to statistical analysis. It was demonstrated that the mutant mLT63 of heat-labile entrotoxin of E.coli constructed in our laboratory devoided of any toxic effect as revealed by the rabbit ileal loop assay, but its adjuvant effect could be demonstrated in the associated immunization of mice with Hp subunit vaccine UreB and Omp11.
