Expressions and significances of microRNA-21, PTEN, NF-κB and caspase-9 in colon carcinoma
10.3760/cma.j.issn.1008-1372.2013.01.013
- VernacularTitle:microRNA-21、PTEN、NF-κB、Caspase-9在结肠癌中的表达及意义
- Author:
Li JIANG
;
Guiying ZHANG
- Publication Type:Journal Article
- Keywords:
MicroRNAs/metabolism;
Genes,tumor suppressor;
NF-kappa B/metabolism;
Caspase 9/metabolism;
Colonic neoplasms/metabolism;
Colonic neoplasms/pathology
- From:
Journal of Chinese Physician
2013;(1):39-41
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate expressions of microRNA-21 (miR-21),PTEN,NF-κB,and caspase-9 in colon carcinoma and their possible mechanisms in progression of tumor.Methods Expressions of above-mentioned indexes and percentage of apoptotic cells in colon carcinoma,colon adenoma,and normal tissue specimen were detected.Results Expressions of miR-21,PTEN,N F-κB,and caspase9 in normal tissue were 0.39 ±0.02,50％,25％,and 50％.Expressions of them in colon adenoma were 0.62 ±0.06,50％,35％,and 55％,and those in colon carcinoma were 0.88 ±0.04,30％,75％,and 20％.Percentage of apoptotic cells were (24.64 ± 7.66) ％,(15.86 ± 1.44) ％,and (6.20 ± 2.57) ％,respectively.The above P-values were all less than 0.05.Expression of miR-21 was positively correlated with TNM stage,depth of infiltration,differentiated level and expression of NF-κB while inversely correlated with PTEN and caspase-9 expressions and percentage of apoptotic cells.The r-values were 0.647,0.297,0.259,0.519,-0.299,-0.388,and-0.931,respectively.Conclusions Hyperexpression of miR-21 participated in progression of colon carcinoma probably through down-regulating expressions of PTEN and caspase-9 and up-regulating expression of NF-κB and then suppressing cell apoptosis.
