Research of the best way to the double-labeling immunofluorescence staining
- VernacularTitle:免疫荧光双重染色样品制备的最佳方法探讨
- Author:
Jipan YAN
;
Yong ZHENG
;
Hao LIU
;
Rui LI
;
Ning ZHANG
;
Weigang CHEN
- Publication Type:Journal Article
- Keywords:
Fixative;
Frozen section;
Immunofluorescence double-labeled
- From:
Journal of Chinese Physician
2012;(z1):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective Detailed descriptions of the double-labeling immunofluorescence staining for fluorescence microscopy provides an ideal sample.Methods Rat liver frozen sections were used fixative were 95％ alcohol,95％ formaldehyde and acetone,frozen sections,with anti-CSE,Ki-67 polyclonal antibody and incubated with FITC,Cy3 fluorescence-labeled secondary fluorescently labeled secondary antibody staining,observed under a fluorescence microscope.Results Acetone fixed group visible in the proliferative phase (S phase) cells showed a red fluorescent nucleus,cytoplasmic green fluorescence.Conclusion The impact of double labeling immunofluorescence the effect of sample links and many factors,including the two most important factors are 2 and coordination with the primary antibody and select the appropriate fixative.
