Protective effect of galanin on DMNQ-induced oxidative stress and cell damage in HEK-293A cells
10.3760/cma.j.issn.1674-6554.2012.12.001
- VernacularTitle:甘丙肽对二甲萘醌引起的人肾胚A型细胞株氧化损伤的保护作用及机制
- Author:
Shu XU
;
Jing SUN
;
Xiaoxiao LI
;
Zhiqing XU
- Publication Type:Journal Article
- Keywords:
Galanin;
Galanin receptors;
DMNQ;
Oxidative stress;
Protection
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2012;(12):1057-1059
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protective effect of galanin on 2,3-Dimethoxy-1,4-naphthoquinone (DMNQ)-induced oxidative stress and cell damage in HEK-293A cells and its possible mechanisms.Methods The expression of galanin and its three receptors (GalR1-3) in HEK-293A were determined with RTPCR technique.The cultured HEK-293A cells were divided into four groups:Control,DMNQ,DMNQ + GAL and DMNQ + AR-M1896 and the cell viability was measured with CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS).Results The RT-PCR data revealed the presence of galanin and its receptors in HEK-293A cells.The expression level was in the order of GalR2 =Galanin ＞ GalR3 ＞ GalR1.DMNQ caused oxidative stress and cell damage in HEK-293A cells with a dose-dependent manner with an IC50 =13.4 μM.Application of galanin reduced the DMNQ-induced cellular toxicity in HEK-293A,which increased cell viability by 24.4％ and 18.8％ in 1 μM and 100 nM,respectively.AR-M1896,an agonist of GalR2 had a similar effect,increased cell viability by 8.7％ and 8.9％ in 1 μM and 100 nM,respectively.Conclusion These data suggest that galanin has a protective effect on DMNQ-induced oxidative stress and cell damage in HEK-293A cells,probably mediated by GalR2.
