The apoptotic activity of hydroxycamptothecin against human gastric carcinoma and its mechanism of action
10.3760/cma.j.issn.1673-4904.2013.11.006
- VernacularTitle:羟喜树碱对胃癌细胞凋亡的影响及其作用机制研究
- Author:
Jiang LIN
;
Ruihua WANG
;
Hui CHEN
;
Xuxiang CHANG
;
Weidong ZHAO
- Publication Type:Journal Article
- Keywords:
Stomach neoplasms;
Apoptosis;
Camptothecin;
Polycomb gene Bmi-1
- From:
Chinese Journal of Postgraduates of Medicine
2013;(11):15-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptotic activity of hydroxycamptothecin (HCFT) against human gastric carcinoma and its mechanism of action.Methods The apoptotic activity of HCPT against gastric carcinoma SGC-7901 cells was detected by MTT assay.The apoptosis occurrence was determined by Annexin V/PI staining and flow cytometry analysis.The mRNA expression of Bmi-1 was detected by real-time PCR analysis.Western blot analysis was used to detect the protein expression of Bmi-1.Results The IC50 values of HCPT against gastric carcinoma SGC-7901 cells was (4.87 ± 0.35) μ mol/L.After treatment with 0,5,10,20 μ mol/L HCPT for 48 h,the percent of apoptosis was elevated from (1.62 ± 0.37)％ to (21.45 ±4.54)％,(36.67 ±5.38)％,(54.26 ±7.14)％ and the 2-△Ct values of Bmi-1 mRNA was decreased from 0.614 ±0.022 to 0.445 ±0.018,0.376 ±0.012,0.215 ±0.010,there had significant difference among the different treatment (P ＜ 0.01).Treated with 0,5,10,20 μ mol/L HCPT for 48 h,the protein expression of Bmi-1 was down regulated by HCPT treatment in a dose-dependent manner by Western blot analysis.Conclusion HCPT shows effective activity to induce apoptosis in gastric carcinoma cells,which is mainly related to the down regulation of Bmi-1.
