Expression of α1-antitrypsin and α1-glycoprotein in children with Wilms tumor and its clinical
10.3760/cma.j.issn.1673-4904.2013.08.011
- VernacularTitle:α1抗胰蛋白酶和α1酸性糖蛋白在儿童肾母细胞瘤中的表达及临床意义
- Author:
Qiuxia FAN
;
Guangyao SHENG
- Publication Type:Journal Article
- Keywords:
Alpha 1-antitrypsin;
Orosomucoid;
Wilms tumor
- From:
Chinese Journal of Postgraduates of Medicine
2013;(8):31-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the serumα1-antitrypsin (α1-AT) and α1-glycoprotein (α 1-AGP) in children with Wilms tumor,and to explore the clinical application value.Methods Thirtyeight children with Wilms tumor who received treatment from January 2007 to December 2010 were designed as observation group,38 healthy children were designed as control group.Fluorescence quantitative PCR was performed to analyze α 1-AT andα 1-AGP mRNA transcription levels of two groups,and ELISA was used to measure the serum concentration of α 1-AT and α 1-AGP.Results The mRNA levels of α 1-AT and α 1-AGP in observation group were 3.50 ± 0.37 and 2.10 ± 0.41,which were significantly higher than those in control group (0.90 ± 0.45,0.50 ± 0.24) (P =0.000,0.002).The levels of α 1-AT and α 1-AGP in observation group were (4516.8 ± 102.4),(1316.0 ± 27.3) g/L,which were significantly higher than those in control group [(2467.4 ± 23.8),(728.6 ± 9.4) g/L] (P =0.015,0.008).Conclusion To detect the levels of α 1-AT and α 1-AGP have the potential value for the diagnosis of Wilms tumor,which can be applied to early screening.