Study of metallo-β-lactamase producing of imipenem resistant Pseudomonas aeruginosa in ICU
10.3760/cma.j.issn.1673-4904.2013.01.005
- VernacularTitle:ICU耐亚胺培南铜绿假单胞菌产金属β-内酰胺酶的研究
- Author:
Shanshan WANG
- Publication Type:Journal Article
- Keywords:
Pseudomonas aeruginosa;
Imipenem;
Drug resistance;
Metallo-β-lactamase
- From:
Chinese Journal of Postgraduates of Medicine
2013;(1):13-15
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the resistance of imipenem resistant Pseudomonas aeruginosa (IRPA),metallo-β-lactamase (MBLs) producing and prevalences of the coding genes of IRPA in ICU.Methods Eighty-six clinical strains of Pseudomonas aeruginosa in ICU continuously collected were analyzed retrospectively.Pseudomonas aeruginosa and its drug susceptibility was identified by BioMerieux VITEK-32 system,and double disk synergy test was used to screen the MBLs producing IRPA.Then,the MBLs phenotype positive IRPA were confirmed by PCR to explore the genotype of them.Results Except polymyxin B with 100.0％ sensitivity and amikacin with higher sensitivity,the percentage of drug resistance for other antibiotics was more than 46％.MBLs phenotype positive IRPA accounted for 48.8％ (42/86) by double disk synergy test and genotype positive accounted for 47.7％(41/86) by PCR in MBLs phenotype positive IRPA.Among these,the most common was IMP-1 which constituted for 26.7％ (23/86).What's more,there were 15.1％(13/86) strains IRPA which carried multidrug resistant genes.Conclusions The main mechanism of drug resistance of IRPA in ICU is MBLs producing and IMP-1 is the most prevalent genotype,and IRPA with multidrug resistant genes is serious.Thus,monitoring of MBLs should be strengthened to help to use antibiotics rationally and prevent transmission of these genes.
