Effects of Fragile Mental Retardation Protein on Cerebellar Neuron Development and Migration in Mouse Model
- VernacularTitle:FMRP影响小鼠小脑皮质神经元发育与迁移的实验研究
- Author:
Xiaohong SUN
;
Yuxia DONG
;
Yu FENG
;
Weike SONG
;
Yue HE
;
Zhiyi HE
- Publication Type:Journal Article
- Keywords:
fragile X mental retardation protein;
deep cerebellar neuclei;
unipolar brush cell;
Purkinje cell
- From:
Journal of China Medical University
2010;(6):420-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of fragile X mental retardation protein（FMRP）on the development and migration of cerebellar neurons in mouse model.Methods Plasmids containing FMRPmutant-EGFP or EGFP were established and transfected into the lateral ventricle of the embryo mouse.Fragile X syndrome（FXS）genotype of the mouse model was identified.Nissl staining and immunofluorescence staining were conducted to assess the changes in neuron development and migration.Results In the experimental group,Nissl staining showed that the deep cerebellar neuclei contracted and divided by white matter,and the non-polarized Purkinje cells retained in internal granular layer;while immunofluorescence staining showed that Tbr2-positive unipolar brush cells changed the migration pathway and accumulated in the ventricular zone.Conclusion Cerebellar neurons showed abnormal formation and migration with the absence of FMRP.
