Polymorphism of Human Cytomegalovirus （HCMV） UL146 Gene
- VernacularTitle:人巨细胞病毒临床株UL146基因多态性研究
- Author:
Shuting CHANG
;
Zhiqin MAO
;
Biao YI
;
Xiaobo YIN
;
Qiang RUAN
;
Rong HE
;
Yaohua JI
;
Zhengrong SUN
;
Yanpingg MA
;
Ying QI
- Publication Type:Journal Article
- Keywords:
Human cytomegalovims;
UL146 gene;
polymorphism;
chemokine
- From:
Journal of China Medical University
2010;(9):749-751
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the polymorphism of human cytomegalovirus（HCMV）UL146 gene in clinical strains,and to evaluate its clinical diagnostic and therapeutic value of gene.Methods The UL146 gene of clinical strains was examined by quantitative polymerase chain reaction（Q-PCR）or general polymerase chain reaction（PCR）.Positive samples of PCR amplification were sequenced and analyzed.Results High variability of UL146 gene was found among 28 HCMV strains.According to phylogenetic analysis,all sequences of UL146 in clinical strains could be divided into three types and four subtypes.Chemokine ELRCXC region was highly conserved in all sequences.Conclusion HCMV-UL146 genes showed a high degree of polymorphism,and its encoded chemokine ELRCXC region was highly con-served.The relationship between HCMV-UL146 gene′s polymorphism and different clinical symptoms of HCMV infection was unclear.
