Biological Characteristics of Human Colon Cancer Cell Line CW-2 Stem Cells
- VernacularTitle:人结肠癌细胞株CW-2干细胞生物学特性研究
- Author:
Mei LIU
;
Liang AI
;
Mi CHEN
;
Hao LONG
;
Shaolin LI
- Publication Type:Journal Article
- Keywords:
cancer stem cell;
colon cancer;
flow-cytometry;
cell isolation;
CD44＋EpCAM＋ cell
- From:
Journal of China Medical University
2010;(6):417-419
- CountryChina
- Language:Chinese
-
Abstract:
Objective To isolate cancer stem cells from human colon cancer cell line CW-2,and observe the biological characteristics.Methods The percentage of CD44＋EpCAM＋ cancer stem cells which were isolated by multi-combination method was evaluated by flow-cytometry.The biological characteristics of the stem cells were analyzed by cell cycle analysis,in vitro invasion assay and in vivo tumorigenicity assay.Results The percentage of CD44＋EpCAM＋ cancer stem cells was 89.57%,and their capabilities of generation,invasion,tumorigenicity were higher than non-cancer stem cells（P 0.05）.Conclusion CD44＋EpCAM＋ cancer stem cells showed low generation,high invasiveness,high tumorigenesis,and they could be isolated by multi-combination.
