Experimental Study of the Apoptosis Inducing Effect of Histamin-indomethacin on Lewis Lung Cancer in Mice
- VernacularTitle:吲哚美辛、组胺-吲哚美辛对鼠Lewis肺癌凋亡诱导作用的实验研究
- Author:
Dong WANG
;
Caixia ZHANG
- Publication Type:Journal Article
- Keywords:
histamin-indomethacin;
Lewis lung cancer;
tumor necrosis factor α;
cell apoptosis
- From:
Journal of China Medical University
2010;(6):435-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis-inducing effect of histamin-indomethacin（his-IN）on Lewis lung cancer cell in mice.Methods The suspension of tumor cells from Lewis lung cancer mice was inoculated into the left axilla of Kunming mice.When the tumor grew to 1 cm in diameter,the mice were divided into 5 groups and received corresponding treatment：3.0 mg/kg indomethacin（IN）group,3.5 mg/kg IN group,3.0 mg/kg his-IN group,3.5 mg/kg his-IN group,and saline control group.Ten days later,the therapeutic effects were evaluated by the tumor inhibitory rate,the apoptotic peak and rate of tumor cells were measured by flow-cytometry（FCM）,and tumor necrosis factor α（TNF-α）level was estimated by radio-immunity method.Results TNF-α level,the tumor inhibitory rate and the apoptosis rate of his-IN group and IN group were significantly higher than those of the control group,and those of 3.5 mg/kg his-IN group was the highest.In the groups of his-IN or IN treatment,an apoptotic peak was found before G0/G1 phase,and the peak in his-IN group appeared earlier than IN group.Conclusion IN and his-IN could resist the growth of the tumor and promote cell apoptosis of Lewis lung cancer in mice.
