Cardiomyocyte Differentiation from P19 Cells Induced by NKX2-5
- VernacularTitle:NKX2-5诱导P19细胞向心肌分化的研究
- Author:
Jinping ZHANG
;
Jun HAO
;
Huijuan WANG
;
Suxia SHAO
;
Duo WANG
;
Yanhong GUO
;
Chunfang ZHAO
;
Lei ZHANG
- Publication Type:Journal Article
- Keywords:
homeobox gene;
Nkx2-5 gene;
P19 cell;
cardiomyocyte differentiation
- From:
Journal of China Medical University
2010;(3):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the rale of NKX2-5 gene in cardiomyocyte differentiation and its mechanism.Methods P19 cells were divided into transfected and non-transfected groups.In the transfected group,P19 cells were with stable expression of NKX2-5 gene.The P19 cells were cultured in suspension for 4 days,and the formed aggregates were transferred to Petri dish for adherent culture.On days 4,8,12,and 16 of the adherent culture,the expressions of ct-saicomeric actin(α-SA)and cardiac troponin T(cTnT)were detected with double-labeling immunofluorescence and Western blot.The ultrastruetural changes were observed on day 16.Results In the transfected group,no expression of α-SA and cTnT was found on day 4,and the expression of these 2 proteins or co-expression existed on days 8,12,and 16.There were early cell junction and myofilament-like structure in the cytoplasm of some cells in the transfected group.In the non-transfected group,these 2 proteins were negative,and no differentiated cell was found.Conclusion Stable expression of NKX2-5 gene can induce cardiomyocyte differentiation from P19 cells,but the P19 cells with stable expression of JVKX2-5 gene is not suitable to be an in vitro model of cardiac development.
