Specific Serum Protein of Colorectal Cancer Detected by Surface-enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry
- VernacularTitle:应用表面增强激光解吸电离飞行时间质谱筛选大肠癌血清特异标志物
- Author:
Ming MA
;
Jianqing SONG
;
Jinming OUYANG
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
surface-enhanced laser desorption/ionization;
time of flight;
mass spectrometry;
weak cation exchanger protein chip;
marker
- From:
Journal of China Medical University
2010;(3):209-211
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the specific serum protein of colorectal cancer by surface-enhanced laser desorption/ionization tune of flight mass spectrometry(SELDI-TOF-MS)and provide the foundation for early detection of colorectal cancer.Methods The levels of serum protein of 36 patients with colorectal cancer and 36 healthy control subjects were detected by weak cation exchanger protein chip(WCX2)chip and SELDI-TOF-MS.Results At the M/Z value range from 2 000 Da to 30 000 Da,82 protein peaks were found.The level of 3 604.240 Da protein was significantly higher in patients with colorectal cancer than in healthy control subjects,while the level of 12 861.368 Da protein was significantly higher in healthy control subjects than in patients with colorectal cancer(P＜0.05).The specificity,sensitivity,and positive predictive value of the detection of colorectal cancer using these 2 protien were 86.11%,77.78%,and 87.5%.respectively.Conclusion The 3 604.240 Da and 12 861.368 Da proteins can be used as the serum marker for early detection of colorectal cancer.
