Application of Tissue-explant Technique in Culturing Rat Submandibular Gland Cells
- VernacularTitle:组织块法培养大鼠颌下腺细胞的实验研究
- Author:
Weiwei HUANG
;
Xuexin TAN
;
Bo LI
;
Zhe WANG
;
Xinyu LI
;
Li MA
;
Dandan HUANG
- Publication Type:Journal Article
- Keywords:
submandibular gland cell;
tissue-explant technique;
enzymatic digestion;
differential adhesion;
tissue engineering
- From:
Journal of China Medical University
2010;(3):194-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of tissue-explant technique in culturing rat submandibular gland cell(RSGC)and the characteristics of RSGC.Methods RSGCs were cultured using tissue-explant technique.The cells were purified by enzymatic digestion and differential adhesion.The cell phenotype was identified by cytokeratin-8 immunohistochemical staining.Cellular morphology was observed and photographed under inverted microscope.The cell viability and growth were determined by a double-staining procedure using FDA-PI and MTT assay,respectively.Results Cytokeratin-8 was positive stained in the immunohistochemical staining.The cell viability was more than 95%.The cell growth curve showed that RSGCs were in logarithmic phase since day 5.Conclusion Tissue-explant technique is an easy way to purify plentiful RSGC with normal functions,and it can be used in further research of tissue engineering of submandibular gland.
