Expression of ABCG2 in Adriamycin-resistant Human Esophageal Cancer Cells
- VernacularTitle:阿霉素诱导食管癌耐药细胞中ABCG2的表达及其意义
- Author:
Liang LIU
;
Jing ZUO
;
Li ZHAO
;
Jing WANG
;
Jianwen GUO
;
Jianghui LIU
;
Lianfu ZUO
- Publication Type:Journal Article
- Keywords:
ATP-binding cassette transporter G2;
flow cytometry;
adriamycin;
esophageal cancer
- From:
Journal of China Medical University
2010;(2):101-104
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of ATP-binding cassette transporter C2(ABCG2) in adriamycin(ADM)-resistant human esophageal cancer cells.Methods The ADM-resistant human esophageal cancer cell(Eca109/ADM) was induced by gradually increasing the ADM concentration in the culture medium of human esophageal cancer cell line(Eca109) and long time screening culture.ABCG2 mRNA and protein of ADM-resistant cells was detected by RT-PCR,flow cytometry(FCM) and Western blot.Drug excretion of Eca109/ADM cells was examined by FCM.The drug resistance index to ADM was detected by MTT.Results The expression of ABCG2 in Eca109/ADM cells was higher than that in Eca109 cells.The drug excretion of Eca109/ADM cells was stronger than Eca109 cells.The Ecal09/ADM cells drug resistance index to ADM was 3.29.Conclusion The ADM-resistant cell line Eca109/ADM was established successfully as an ideal chemoresistant cell model.ABCG2 might be involved in the drug resistance of esophageal cancer cell.
