Effect of Wild Type p53 Gene on Inhibiting the Centrosome Hyperamplification in Bladder Cancer Cell Line
- VernacularTitle:野生型p53基因调控膀胱移行细胞癌中心体异常的实验研究
- Author:
Ming WANG
;
Lianqu WANG
;
Yi TAN
- Publication Type:Journal Article
- Keywords:
wild type p53 gene;
bladder cancer cell line;
centrosome hyperamplification;
chromosome instability
- From:
Journal of China Medical University
2010;(2):128-131
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of wild type p53 gene on centrosome hyperamplification in bladder cancer cells.Methods A wild type p53 gene recombinant adenovirus vector AdCMVp53 was constructed,and then trangfected into the human bladder cancer cell line T24.The cells were stained with the monoclonal antibody against pericentrin by indirect immunofluorescence method.The change of centrosome hyperamplification was observed under the fluorescence microspcope.Results Introduction of wild type p53 could suppress the centrosome amplification of T24 cell line.Conclusion p53 might play an important role in the regulation of centrosome hyperamplification.The loss of p53 might be one of the mechanisms involved in chromosome instability and contribute to the genesis and development of the bladder carcinoma.
