Effects of Aspirin on the Proliferation and Apoptosis of Human Endometrial Adenocarcinoma Ishikawa Cells
- VernacularTitle:阿司匹林对人子宫内膜腺癌Ishikawa细胞生长和凋亡的影响
- Author:
Dongni ZHAO
;
Yingnan JIN
;
Yanxia LI
;
Xiaoxin MA
- Publication Type:Journal Article
- Keywords:
aspirin;
Ishikawa cell;
tumor growth;
endometrial adenocarcinoma
- From:
Journal of China Medical University
2010;(2):132-134
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the effects of aspirin on the proliferation and apoptosis of human endometrial adenocarcinoma cells.Methods MTT assay was used to measure the effects of aspirin on the proliferation of Ishikawa cell.Flow cytometry(FCM) was employed to examine the distribution of cell cycles and the rates of apoptosis.Transmission electron microscopy was used to observe cell morphologic changes after aspirin administration.Results Aspirin inhibited the proliferation of cultured Ishikawa cells in a time-dependent and dose dependent manner(P<0.05).Aspirin increased the distribution of G,stage and the rates of cell apoptosis in a dose-dependent manner(P<0.05).Morphologic features of apoptosis cells,including cell shrinkage,nuclear condensation and apoptotic bodies could be found obviouslyunder the transmission electron microscopy.Conclusion Aspirin inhibited the proliferation and increased the apoptosis of human endometrial adenocarcinoma Ishikawa cells.
