Effects of Aerobic Exercise on Calcium Release Related Genes Expression in Sarcoplasmic Reficulum of Skeletal Muscle in Mice
- VernacularTitle:有氧运动对C57BL/6小鼠骨骼肌肌浆网钙释放相关基因表达的影响
- Author:
Xiaolei LIU
;
Yanmei NIU
;
Hairui YUAN
;
Sujuan LIU
;
Li FU
- Publication Type:Journal Article
- Keywords:
aerobic exercise;
sarcoplasmic reticulum;
calcium signaling pathway;
ryanodine receptor1;
phospholi-pase C-delta 4
- From:Chinese Journal of Sports Medicine
2010;(3):299-304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of 6-week aerobic treadmill exercise on the genes expression in skeletal muscle of C57BL/6 mice.Gene array was performed and the data was analyzed to evaluate the adaptive changes at gene level caused by exercise.Methods Twenty male C57BL/6 mice were randomly divided into two groups:control group(C) and aerobic exercise group (E).The mice from group E were forced to run on treadmill for 6 weeks.After 6 weeks,all mice were fasted for 16 hours,and then sacrificed.Their flexor muscle was removed.4 samples from each group were analyzed with gene chips to dissect the differentially expressed genes between the two groups.Results Our results showed that 723 genes were differentially expressed in E group as compared with C group after the 6 weeks aerobic exercise,among which 510 genes were up-and 213 down-regulated.There were 6 differentially expressed calcium signaling genes and 2 ofwhich(Gnall and Pdgfra) were up-regulated,while the others(Phka1,Picd4,RYR1 and Ppid)were down regulated.
