A Preliminary Study on Antitumor Effect of Specific Cytotoxic T Lymphocytes Activated by Exosomes Secreted from Dendritic Cell Against Renal Carcinoma
- VernacularTitle:树突状细胞分泌的exosomes体外诱导特异性CTL抗肾癌的初步研究
- Author:
Xinli WANG
;
Qing LI
;
Ting GAO
;
Liye FU
;
Yang WANG
;
Tao JIANG
;
Youhong JIANG
- Publication Type:Journal Article
- Keywords:
dendritic cell;
exosome;
cytotoxic T lymphocyte;
renal carcinoma
- From:
Journal of China Medical University
2010;(1):4-6
- CountryChina
- Language:Chinese
-
Abstract:
Objective To extract exosomes from dendritic cell (DC)and investigate antitumor effect of the special cytotoxic T lymphocytes activated by exosomes against 769-P.Methods Monocytes from peripheral blood of healthy donors were cultured to induce DC in intro and their phenotypes were analyzed by FACS.The exosomes were extracted from DC by ultrafiltration and ultracentrifugation and observed under electric microscope.Proliferation of T cells and the cytotoxicities of CTL against 769-P induced by exosome were measured by MTT.Results The exosomes could be separated from culture supernatant of DC by ultrafiltration and ultracentrifugation.The combination of the exosomes from DC and the 769-P antigen could effectively stimulate T cell proliferation and enhance their cytotoxicity, the absorption value of T cell proliferation(1.68±0.22), the cytotoxicity of CTL(38.23±2.16)%.Conclusion The exosomes extracted by ultrafiltration and ultracentrifugation can present tumor antigen to T cells and induce the cytotoxicity of CTL.
