Effect of MAPKK/MEK-MAPK/ERK Signal Transduction Pathway in Rat Neural Stem Cells
- VernacularTitle:大鼠神经干细胞中MAPKK/MEK-MAPK/ERK信号转导通路的实验研究
- Author:
Yu ZHAO
;
Peng XIE
;
Xiaofen ZHU
;
Zhiyou CAI
- Publication Type:Journal Article
- Keywords:
mitogen activated protein kinases kinases;
extracellular signal regulated kinase;
neural stem cell
- From:
Journal of China Medical University
2010;(1):14-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To incubate the rat neural stem cells with specific inhibitor (PD98059) of MAPKK/MEK to clarify the effect of MAPKK/MEK-MAPK/ERK signaling pathway in neural stem cells (NSC).Methods NSCs derived fiom E15-16 rats were isolated and cultured.After treated with different concentration of PD98059,they were subjected to the detection of cell proliferation,Nesn'n, BrdU and β-tubulin-Ⅲ by WTS-8 assay,immunofluorescent staining or Western Blot respectively.Results PD98059 had an effect on the survival,proliferation and differentiation of NSC at a concentration-dependent manner.The viability of the NSC was significantly decreased than the control (P＜0.05),whereas the numbers of BrdU-positive and β-tubulin-Ⅲ positive cells were notably fewer than the control (P＜0.05) after incubated with the higher concentration of PD98059.The ERK expression was blocked by PD98059 at a concentration-dependent manner.Conclusion The MAPKK/MEK-MAPK/ERK signaling pathway played a vital role in the survival, proliferation and differentiation of NSC.
