Effect of 5-Fluorouracil and Radiotherapy on the Apoptosis of Human Cervical Adenocarcinoma HeLa Cells
- VernacularTitle:5-FU和放射线联合应用对人子宫颈腺癌HeLa细胞凋亡的影响
- Author:
Bin YANG
;
Min CAI
;
Hua YAN
;
Huawei ZHOU
;
Fan YANG
;
Yuan LIU
- Publication Type:Journal Article
- Keywords:
5-fluorouracil;
radiotherapy;
HeLa cells;
apoptosis
- From:
Journal of China Medical University
2010;(1):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 5-fluorouracil (5-FU) combined with radiotherapy (RT) on the apoptosis of human cervical cancer HeLa cells.Methods The HeLa cells were divided into four groups treated with chemotherapy(ChT),RT,RT+ChT, and control groups.The non-cytotoxic concentration (48 h IC_(50)) of HeLa cells treated with 5-FU was determined by methyl thiazolyl tetrazolium (MTT) and the apoptosis rates of HeLa cells detected by flow cytometry with annexin V-FTTC and PI double labeling.The morphologic changes of the apoptosis cells were observed under fluorescence microsope.Results ChT,RT and RT+ChT treatment could induce the apoptosis of HeLa cells with the strong induction of the combined treatment (P＜0.05).Conclusion 5-FU combined with RT can obviously enhance the apoptosis of HeLa cells, which provides a reliable experimental evidence for clinical use of 5-FU combined with RT in the intervention of human cervical cancer.
