Effect of Exercise Preconditioning on the Expression of Calcitonin Gene-Related Peptide in the Dorsal Root Ganglion of Rats
- VernacularTitle:运动预适应对大鼠背根神经节降钙素基因相关肽表达的影响
- Author:
Shanshan PAN
;
Xiaojuan SUN
- Publication Type:Journal Article
- Keywords:
exercise preconditioning;
ischemic preconditioning;
calcitonin gene-related peptide;
dorsal root ganglion;
rat
- From:
Chinese Journal of Sports Medicine
2010;(1):30-33
- CountryChina
- Language:Chinese
-
Abstract:
Objective The goal of this study was to explore the effect of exercise preconditioning on the expression of calcitonin gene-related peptide(CGRP)in the dorsal root ganglion of rats.Methods Fifty healthy male SD rats were randomly divided into control group (C) and exercise preconditioning group(EP).Group EP performed intervial treadmill exercise for 3 weeks for establishing exercise preconditioning animal model.The expression of CGRP mRNA in dorsal root ganglion was investigated by real-time fluorescence quantitative PCR.The immunoreaetion of CGRP in dorsal root ganglion was shown by immunohistochemistry.Results There was no significant difference in the expression of CGRP mR.NA in two groups(P>0.05).As compared with the group C,the immunoreaction of CGRP was increased in group EP,and the positive area and mean optical density of CGRP immunoreaction in group PE were significant higher than that in group C(P<0.05).Conclusion Exercise preconditioning does not change the expression of CGRP mRNA in dorsal root ganglion,but enhances the expression of CGRP in dorsal root ganglion to promote the reserve and release of CGRP in peripheral nerve endings and has the same endogenous protection as ischemie preconditioning.
