Proteome Analysis of Hirschsprung Disease by Two-dimensional Electrophoresis
- VernacularTitle:先天性巨结肠组织蛋白质组成分的双向凝胶电泳分析
- Author:
Hong GAO
;
Liangying LU
;
Weiliang BAI
;
Dajia WANG
;
Ying HUANG
;
Zhibo ZHANG
;
Weilin WANG
- Publication Type:Journal Article
- Keywords:
Hirschspning disease;
proteomics;
two-dimensional polyacrylamide gel electrophoresis;
differential expression
- From:
Journal of China Medical University
2009;(10):761-763
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the protein expression profiles in stegnotic and normal segment of Hirschspning disease (HD) and find the differentially expressed proteins. Methods Immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to isolate the proteins from stegnotic and normal segment of HD. After the samples were treated with silver staining,ImageMaster 2D Platinum analysis software was used to analyze the differentially expressed proteins. Results Repeatable 2-DE profiles were obtained. The mean matching rates of the stegnotic and normal mucosa were 78.1% and 86.7%,respectively. Totally, 103 spots of differentially expressed proteins were screened out between the stegnotic and the normal segment of HD. Conclusion Good reproduuibility and resolution could be obtained in the tissues of HD by applying immobilized pH gradient two-dimensional polyacrylamide gel electrophoresis.Screened differentially expressed proteins may provide the candidates of the markers for early detection of HD.
