Detection of BJ-TSA-9, LUNX and CK19 mRNA in Peripheral Blood and Its Diagnostic Value in Lung Cancer
- VernacularTitle:肺癌患者外周血BJ-TSA-9,LUNX和CK19 mRNA的检测及其临床意义
- Author:
Penghui LIU
;
Guoqing LIAO
;
Yuemin LI
;
Yanhui YIN
;
Hongmei WANG
;
Yimei QU
- Publication Type:Journal Article
- Keywords:
lung cancer;
polymerase chain reaction;
peripheral blood
- From:
Journal of China Medical University
2009;(10):786-789
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the detection of BJ-75A-9 mHNA, WNX and CK19 mRNA in peripheral blood cells of lung cancer patients and evaluate its diagnostic value. Methods The expression of BJ-TSA-9, LUNX and CK19 mRNA in peripheral blood cells was delected from 84 lung cancer patients, 32 benign lung lesions and 20 healthy volunteers by nested reverse transcription polymerase chain re-action (Nested-RT-PCR). Results The positive detection rates of BJ-75A-9,LUNX and CK19 mRNA in the peripheral blood of the pa-tients with lung cancer were 59.5%,40.4%and 22.6% respectively. In the 32 peripheral blood samples of the patients with benign lung le-sions,the positive detection rate of BJ-TSA-9,LUNX and CK19 mRNA were 9.3%, 12.5% and 6.3% respectively. No expression of BJ-TSA-9,LUNX and CK19 was detected in lhe samples of the healthy volunteers. The expression level of BJ-TSA-9, WNX and CKI9 mRNA in the peripheral blood of lung cancer stage IV patients was significantly higher than those of stages II and ID (P< 0.05). Conclusion RT-PCR amplification of BJ-TSA-9, LUNX and CK19 mRNA are an efficient way to detect early haematogenous dissemination of cancer cells for lung cancer patients. The detection of BJ-TSA-9 expression is sensitive and specific for lung cancer,and it is superior to both LUNX and CK19 mRNA. The expression of BJ-7SA-9 mRNA in peripheral blood might predict the hematogenous metastatic spreading of lung cancer cells.
