Expression of cytoglobin gene in hypoxic-ischemic brain damage of newborn rat

VernacularTitle:细胞红蛋白基因在新生大鼠缺氧缺血性脑损伤时的表达
Author: Hanhua YANG ; Shufeng TIAN ; Xuechuan SHI ; Danping XIAO
Publication Type:Journal Article
Keywords: Cytoglobin; Newborn Rats; Gene expression; Hypoxic-ischemic brain damage
From: International Journal of Pediatrics 2013;(2):208-210
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression and change of cytoglobin(Cygb)gene on hypoxicischemic brain damage(HIBD).Methods Fifty SD rats of 7days old were divided into four study groups and one control group.The brain tissues were taken at 4hours,12hours,24hours and 48hours after the onset of HIBD.Cygb mRNA was determined by the reverse transcription PCR.One-way method of GraphPad Prism was used for statistics.Results The fragment length of PCR products was identical with experimental design.The expression level of Cygb gene increased at 4h after ischemia,and peaked at 24h.48h after HIBD,the Cygb gene level began to decrease.Conclusion The expression of Cygb in brain tissue increased rapidly after HIBD of newborn rats,suggesting that Cygb may have important function in the protection process of HIBD.