Expression and methylation of TCF21 gene in non-small cell lung cancer
10.3760/cma.j.issn.1001-4497.2013.01.007
- VernacularTitle:非小细胞肺癌TCF21基因表达及其启动子区甲基化的研究
- Author:
Wenwu HE
;
Song HU
;
Mingwu CHEN
;
Nuo YANG
;
Lei XIAN
- Publication Type:Journal Article
- Keywords:
Transcription factor 21;
Western-blot;
Methylation;
Carcinoma,non-small-cell lung
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2013;(1):24-27
- CountryChina
- Language:Chinese
-
Abstract:
Objective Background and objective Bioinformatics technology found the TCF21 gene has difference expression in non-small cell lung cancer (NSCLC) and benign lung tissue.To explore TCF21 mRNA and protein expression in nonsmall cell lung cancer and its methylation of the promoter region is the aim of this study.Methods Use RT-PCR,Western blot and Pyrosequencing detected TCF21 gene mRNA,protein and the methylation of the promoter region respectively in 97 cases of non-small cell lung cancer and 21 cases of benign lung tissue.Results TCF21 gene mRNA-positive expression were detected 23 cases (23.71％) and 14 cases (66.67％) in 97 cases of NSCLC cancer tissue and 21 cases of benign lung tissue,the average gray value of TCF21 protein expression levels in NSCLC cancer tissue is 0.49 ± 1.78,while it is 1.48 ± 1.58 in benign lung tissue,the TCF21 gene promoter region have varying degrees methylation in NSCLC cancer tissue and benign lung tissue,and the significant of methylation frequency was found statistically significant between NSCLC cancer tissue and benign lung tissue,also it has higher frequency of 49.04％ and 51.37％ respectively at point 1 and 5 in NSCLC cancer organizations.Conclusion TCF21 gene mRNA and protein expression were statistically significant in NSCLC cancer tissue and benign lung tissue,the TCF21 gene promoter region average methylation frequency was significantly higher than that in benign lung tissue cells.
