Effects of Nonylphenol on L-type Ca~(2+) Currents in Isolated Guinea-pig Ventricular Myocytes
- VernacularTitle:壬基苯酚对豚鼠心室肌细胞L-型钙电流的影响
- Author:
Qinghua GAO
;
Tong ZHU
;
Feng GUO
;
Lei YANG
;
Meimi ZHAO
;
Gang LI
;
Liying HAO
- Publication Type:Journal Article
- Keywords:
nonylphenol;
L-type Ca~(2+)currents;
patch clamp technique
- From:
Journal of China Medical University
2010;(2):81-83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of nonylphenol(NP) on L-type Ca~(2+) currents(I_(Ca-L)) in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca~(2+) currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited lew,at different potentials.Nonylphenol (10~(-6) mol·L~(-1),10~(-5) mol·L~(-1)) reduced the peak amplitude of I_(Ca-L) from-3.2±1.5 pA·pF~(-1) to-1.6±0.8 pA·pF~(-1)(P<0.01) and-1.4±0.7 pA·pF~(-1)(P<0.01),respectively.Nonylphenol did not influence the activation curve of I_(Ca-L) significantly.Conclusion Nonylphenol could in hibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner.Objective To observe the effects of nonylphenol(NP) on L-type Ca~(2+) currents(I_(Ca-L))in isolated guinea-pig ventricular myocytes.Methods The ventricular myocytes were isolated from guinea-pig hearts.The L-type Ca~(2+) currents in the ventricular myocytes treated with NP were measured with whole cell patch-clamp technique.Results Nonylphenol inhibited lew,at different potentials.Nonylphenol hibit the L-type calcium currents in isolated guinea-pig ventricular myocytes with a concentration-dependent manner.
