Preparation and Evaluation of Long-circulating Liposomes of Paraoxonase
- VernacularTitle:对氧磷酶长循环脂质体的制备及评价
- Author:
Zhenkun HAN
;
Jianbo SUN
;
Dan LIU
;
Haiyang HU
;
Dawei CHEN
;
Pengyi GU
;
Min ZHAO
- Publication Type:Journal Article
- Keywords:
paraoxonase;
long-circulating liposome;
film dispersion method;
protein;
partical size distribution
- From:
Journal of China Medical University
2010;(2):87-91
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the long-circulating liposomes of paraoxonase(PON).Methods The long-circulating liposomes of paraoxonase were prepared by film dispersion method.The encapsulation efficiency was determined by gel column.The effects of the factors on the encapsulation efficiency,such as the weight ratio of paraoxonase to phospholipid,cholesterol(Choi) to phospholipid,PEG-cholesterol (PEG-Chol) and the iron strength of water phase,were investigated respectively.Then the formulation was optimized by orthogonal design.Results The encapsulation efficiency of the paraoxonase liposomes was 87.66±3.46%,and the average diameter of the liposomes was about 126 nm.There was no significant change on encapsulation efficiency on 15 d at 4 ℃,and the activity of paraoxonase was maintained basically stable.Conclusion The preparation of PEG-modified paraoxonase liposomes was easy and practicable,and the property investigation in vitro showed that the paraoxonase liposomes were stable.
