The Relationship between the MICA/B Expression on Cancer Cell Lines and Their Sensitivity to NK Cell Cytotoxicity
- VernacularTitle:NK细胞杀瘤活性与肿瘤细胞表面MICA/B表达的相关性研究
- Author:
Xinli WANG
;
Qing LI
;
Tao JIANG
;
Yang WANG
;
Liye FU
;
Ting GAO
;
Youhong JIANG
- Publication Type:Journal Article
- Keywords:
natural killer cell;
tumor cell lines;
MICA/B;
cytotoxicity
- From:
Journal of China Medical University
2010;(2):95-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relationship between the expression of MHC class I chain-related gene A/B(MICA/B) on different human cancer cells and their sensitivity to NK cell cytotoxicity.Methods Hie expression MICA/B in K562,Bcap37,769P and A549 cells was measured by FACS.Isolation of NK cells were obtained by the modified RosetteSep~((R)) procedure.Cytotoxicity of NK cells at different ef0.50)%,(90.72±0.64)%,(55.59±0.55)%,and(3.84±0.10)% respectively.The purity of NK cells obtained by the modified RosetteSep~((R)) procedure was(96.52±2.42)%,whereas the cytotoxicity of NK cells against K562,Bcap39 and 769P was much higher than that of A549(P<0.01).The expression of MICA/B was significantly correlated with the cytotoxicity of NK cells at E:T ratios of 5:1,10:1 and 20:1 respectively(P<0.01).Conclusion The MICA/B expression on cancer cell lines was correlated with NK cell-mediated cytolysis and influenced the cytotoxicity of NK cells.
