mTOR in Ischemic Postconditioning-induced Attenuation of Lschemia/reperfusion Injury in Rat Skeletal Muscle
- VernacularTitle:雷帕霉素靶蛋白参与缺血后处理减轻大鼠骨骼肌缺血/再灌注损伤
- Author:
Jun YANG
;
Xinhua HU
;
Zhishen ZHANG
;
Chengwei LIU
;
Zhenggui YI
;
Qiang ZHANG
- Publication Type:Journal Article
- Keywords:
mammalian target of rapamycin;
ischemic postconditioning;
ischemic preconditioning;
skeletal muscle
- From:
Journal of China Medical University
2010;(3):178-180
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of mammalian target of rapamycin(mTOR)in ischemic postconditioning(I-postC)-induced attenuation of ischemia/reperfusion(I/R)injury in rat skeletal muscle.Methods A total of 48 healthy male Wistar rats were randomly divided into 3 groups(n=16 each group):I/R group(4-hour ischemia followed by 12-or 24-hour reperfusion),ischemic preconditioning (IPC)group(3 cycles of 5-minute ischemia followed by 5-minute reperfusion),and I-postC group(3 cycles of 1-minute reperfusion followed by 1-minute ischemia).The rat model of I/R injury in right hind limb model was established by clamping the right femoral artery.The changes in the morphology,wet-to-dry weight ratio(W/D),malondialdehyde(MDA),and myeloperoxidase(MPO)in skeletal muscle were compared.The expression of mTOR was detected by Western blot and immunohistochemistry.Results In I-postC and IPC groups,the skeletal muscle edema was less severe,the levels of MDA and MPO significantly decreased,and the expression of mTOR significantly in creased,compared with I/R group(all P＜0.03).There was no significant difference between I-postC and IPC groups.Conclusion Ipostc may attenuate I/R injury in rat hind limbs by activating mTOR signal pathway,which is similar to the mechanism of IPC.
