Expression and Characterization of the Recombinant Epitopes of HSV-gB and HSV-gD Protein
- VernacularTitle:HSV-gB、gD糖蛋白重组串联抗原表位蛋白的表达及鉴定
- Author:
Jingyi GAO
;
Yue WANG
;
Yuji ZHAO
;
Kai FANG
;
Jinsong ZHANG
- Publication Type:Journal Article
- Keywords:
herpes simplex virus;
gB glycoprotein;
gD glycoprotein;
epitope;
antigen
- From:
Journal of China Medical University
2010;(3):181-183
- CountryChina
- Language:Chinese
-
Abstract:
Objective To prepare the recombinant epitopes of HSV-gB and HSV-gD protein and provides a new antigen protein for the development of herpes simplex virus(HSV)vaccine.Methods The epitopes of HSV-gB and HSV-gD protein were analyzed by epitope prediction software.A novel gene named X which encoded 9 predicted epitopes of HSV-Gb and HSV-gD protein was designed and synthesized using chemical method.X gene was cloned into vector PET-28a(+),expressed in Escherichia cob' BL21(DE3),and analyzed by Western blot.Results X gene was successfully designed and expressed in Escherichia coli BL21(DE3).Western blot analysis showed that recombinant X protein,which was with His marker,can be detected by anti-His antibody.Conclusion In this study we establish a newmethod to express recombinant epitope protein,which may be a new protein for developing vaccine against HSV infection.
