Caveolin-1 inhibits vascular anastomotic restenosis by downregulating ERK1/2
10.3760/cma.j.issn.1007-631X.2013.02.018
- VernacularTitle:Caveolin-1下调细胞外调节蛋白激酶1/2抑制血管吻合口再狭窄
- Author:
Guozuo XIONG
;
Guoshan BI
;
Xianpeng DAI
;
Xin SHEN
;
Liming DENG
;
Jie JIANG
;
Hui LIU
- Publication Type:Journal Article
- Keywords:
Extracellular signal-regulated MAP kinases;
Disease models,animal
- From:
Chinese Journal of General Surgery
2013;(2):142-145
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Caveolin-1 on extracellular regulated protein kinases of rabbit carotid artery anastomotic restenosis.Methods 40 New Zealand white rabbits were randomly divided into normal control group,carotid artery end to end anastomosis surgical group,empty vector transfection on the site of anastomosis group and Caveolin-1 transfected group.Left carotid artery endto-end anastomosis was performed,and the mixture of Caveolin-1 plasmid and liposome lipofectin 2000 (transfected group) or empty plasmid and lipofectin 2000 mixture (empty vector group) were transfected on anastomosis.Specimens were taken at 7 d after surgery for Western blot and RT-PCR to detect the expression of protein and mRNA.Specimens were taken for HE staining at 28 d to observe the proliferation of intima,and measured the ratio of intima/media area by Image-Pro Plus 6.0 software.Results Compared with surgical group,the ratio of intima/media area in Caveolin-1 transfected group decreased by about 50％.Compared with surgical group and empty vector group,the Caveolin-1 mRNA expression and protein activity significantly increased (t =36.59,P ＜ 0.01) ; the ERK1/2 mRNA expression and protein activity significantly decreased on rabbit carotid artery anastomotic site in Caveolin-1 transfected group (t =32.64and 7.27,P ＜ 0.01).Conclusions Caveolin-1 inhibits anastomotic restenosis possibly by regulating the activation of ERK.
