Ulinastai reverses experimental hepatofibrosis in rats
10.3760/cma.j.issn.1007-631X.2013.02.017
- VernacularTitle:乌司他丁逆转大鼠肝纤维化的实验研究
- Author:
Weili GU
;
Shuai ZHANG
;
Liang SHEN
;
Di HUANG
;
Jiefeng WENG
- Publication Type:Journal Article
- Keywords:
Liver cirrhosis;
Transforming growth factor beta1;
Caspase-3;
Ulinastai
- From:
Chinese Journal of General Surgery
2013;(2):138-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore if Ulinastai reverses hepatofibrosis in rats.Method Rat hepatofibrosis models were induced by TAA subcutaneously injection.18 rats with hepatofibrosis were divided into 2 groups,Ulinastai treatment group (9 rats),normal saline control group (9 rats).AST,ALT,HA,SOD,MDA,LN level were measured and compared between the 2 groups and that of healthy rats (3 rats).Rats liver morphology was observed using HE,Masson stain and type-B ultrasonic.TGF-β1、caspase-3 were detected by immunohistochemistry.Result After 3 weeks Ulinastai treatment,elastic index and SSS score in Ulinastai group decreased significantly compared to pretreatment (t =2.472,P ＜0.05).ALT,AST,SOD,MDA,HA and LN level significantly improved (F =3.862,5.774,3.442,4.157,4.173,3.674,P ＜ 0.05).Compared with NS treatment group HE and Masson staining showed fewer inflammatory cells infiltration in central vein,ballooning degeneration of liver cells,collagen proliferated,hepatic lobules degradation and pseudolobule arise after 3 weeks intervenion.Also in Ulinastai group,TGF-β1、caspase-3 positive cells were much less than that in NS treatment group.Conclusions Ulinastai can reverse rat hepatic fibrosis and alleviate fibrosis degree and collagen fiber deposit.
