A screening study for a novel tumor suppressor gene CSRP1 in Chinese origin patients with sporadic colorectal cancer
10.3760/cma.j.issn.1007-631X.2013.02.009
- VernacularTitle:散发性结直肠癌相关半胱氨酸甘氨酸富集蛋白1抑癌基因的筛选
- Author:
Chongzhi ZHOU
;
Yang HAN
;
Xiaoliang WANG
;
Guoqiang QIU
;
Zhihai PENG
- Publication Type:Journal Article
- Keywords:
Colorectal neoplasms;
Genes,tumor suppressor;
Gene expression
- From:
Chinese Journal of General Surgery
2013;(2):108-111
- CountryChina
- Language:Chinese
-
Abstract:
Objective In previous study,we carried out refined mapping of loss of heterzygosity (LOH) on 1q31.1-32.1 and found that a minimal region of frequent deletion was located at DIS413-D1S2622,which indicated that the region could harbor a tumor suppressor gene associated with colorectal carcinoma.This study was to screen for the potential tumor suppressor gene (TSG) on D1S413-D1S2622 in Chinese origin patients with sporadic colorectal cancer.Methods 25 genes located in the D1S413-D1S2622 region were chosen and a microarray-based high throughput screening conducted in 19 sporadic colorectal cancers to identify candidate tumor suppressor genes.The relationship between expression levels of candidate genes and the clinicopathological data was analyzed.Real-time PCR was performed to validate the microarray results.Results According to the microarray-based high throughput screening,we found 4 significantly down-expressed genes,including CSRP1,LMOD1,PPP1R12B and CFHL3.There was no significant association between of CFHL3,CSRP1,LMOD1,PPP1R12B expression and the clinicopathological data.CSRP1 could be a colorectal cancer related tumor suppressor gene.CSRP1 was down-regulated in colorectal cancer.Conclusions CSRP1 might be involved in the progression of colorectal cancer.
