The association of killer cell immunoglobulin like receptor gene polymorphism with cytomegalovirus infection after hematopoietic stem cell transplantation
10.3760/cma.j.issn.0578-1426.2013.02.026
- VernacularTitle:杀伤细胞免疫球蛋白样受体基因多态性对造血干细胞移植后巨细胞病毒感染的影响
- Author:
Xiaojin WU
;
Jun HE
;
Depei WU
;
Xiaojing BAO
;
Chao XU
;
Huifen ZHOU
;
Aining SUN
;
Yue HAN
;
Xiaowen TANG
;
Zhengzheng FU
;
Xiao MA
- Publication Type:Journal Article
- Keywords:
Hematopoietic stem cell transplantation;
Cytomegalovirus;
KIR genotype
- From:
Chinese Journal of Internal Medicine
2013;(2):161-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the influence of the killer cell immunoglobulin like receptor (KIR) gene polymorphism on cytomegalovirus (CMV) infection and pathogenesis after hematopoietic stem cell transplantation (HSCT).Methods The KIR genotype was determined by sequence-specific primer polymerase chain reaction (PCR-SSP) in 138 pairs of donors and recipients before HSCT during October,2005 and May,2011.Posttransplant monitoring for CMVpp65 antigen was performed by indirect immune histochemically assays since week 2 after transplantation.The differences between CMV positive group and negative group,inhibitive and active KIR of donors and recipients,and KIR haplotype frequency of donors and recipients were analyzed.Results There were no significant differences in frequency of KIR gene and haplotype AA,AB,BB between the donors and recipients.The frequencies of 2DS2 and 2DS4 * 003-007 of donors in CMV positive group were obviously lower than those in CMV negative group with significant differences(8％ vs 16％,P =0.0420;3％ vs 13％,P =0.0050).There was no significant difference in KIR gene between CMV positive group and CMV negative group.The CMV infection rates of haplotype AA,BB,AB donors were 64.38％,36.84％ and 50.00％,while CMV infection rates of haplotype AA,BB,AB recipients were 53.73％,46.15％ and 51.72％,respectively.The CMV infection rate was higher in the patients received KIR haplotype AA donor than in those received KIR haplotype BB donor (36.84％ vs 64.38％,P =0.0299).2DS4 * 003-007 and haplotype BB of donor were found associated with CMV infection in multifactor analysis.Conclusion KIR genotypes of donors are associated with CMV infection after HSCT.
