Detection of point mutations in genes related to fluconazole resistance in Candida albicans

VernacularTitle:白念珠菌耐氟康唑相关基因点突变的检测
Author: Weifang JING ; Chunli LI ; Yali GUO ; Yuanjun LIU ; Yuqing QI ; Quanzhong LIU ; Huiping WANG
Publication Type:Journal Article
From: Chinese Journal of Dermatology 2013;(3):196-197
CountryChina
Language:Chinese
Abstract: Objective To investigate the relationship of point mutations in ERG11,TAC1,MRR1 and UPC2 genes to fluconazole resistance in Candida albicans.Methods This study included 11 fluconazole-resistant Candida albicans strains.DNA was extracted from these Candida isolates by urea pyrolysis method.PCR was carried out to amplify the full-length EGR11 gene,as well as the corresponding fragments of TAC1,MRR1 and UPC2 genes followed by DNA sequencing.Results Eight mutations were detected in the ERG11 gene,including D116E,Y132H,Y132F,K143Q,T229A,E266D,G448E and G464S.Among them,K143Q was a novel mutation and had not been reported.A G648D mutation was detected in the UpC2 gene.No mutations were detected in the TAC1 or MRR1 gene.Conclusions There is a definite association between fluconazole resistance and ERG11 mutations,while further research is warranted to clarify the relationship between the mutations of TAC1,MRR1,UPC2 genes and resistance to fluconazole.