Relationship between the valine/leucine247 polymorphism of apolipoprotein H gene and generation of antiphospholipid antibodies in patients of Han nationality with systemic lupus erythematosus in Wenzhou region
10.3760/cma.j.issn.0412-4030.2013.01.007
- VernacularTitle:温州汉族SLE患者载脂蛋白H基因Leu247Val多态性与抗磷脂抗体的相关性研究
- Author:
Liqing ZHU
;
Haixiao XIE
;
Lihong YANG
;
Yanhui JIN
;
Wangqiang HU
;
Mingshan WANG
;
Hongying SHI
;
Pengfei XU
;
Xiaochun ZHU
- Publication Type:Journal Article
- Keywords:
Lupus erythematosus,systemic;
beta 2-glycoprotein I;
Genes;
Lupus coagulation inhibitor
- From:
Chinese Journal of Dermatology
2013;(1):16-19
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the valine/leucine247 (817G/T) polymorphism in exon 7 of apolipoprotein H (apoH) gene and the generation of antiphospholipid (APL)antibodies in patients of Han nationality with systemic lupus erythematosus (SLE) in Wenzhou region.Methods This study included 165 patients with SLE and 160 healthy controls of Han nationality in Wenzhou region.Venous blood samples were obtained from all of the subjects followed by the isolation of blood plasma,sera and white blood cells.PCR and DNA sequencing were carried out to assess the Leu/Va1247 polymorphism in apoH gene.Lupus anticoagulant (LAC) was detected by Russell viper venom time (RVVT) assay.Enzyme-linked immunosorbent assay (ELISA) was carried out to quantify the serum levels of anti-β2-glycoprotein Ⅰ (GPI) antibodies and anticardiolipin antibodies (ACA).Chi-square test was carried out to compare the 817G/T polymorphism between the patients and controls,and Logistic regression analysis to evaluate the correlation between the 817G/T polymorphism and production of antiphospholipid antibodies.Results There were significant differences between the patients and controls in the genotype distribution and allele frequency at position 817 of apoH gene (both P ＜ 0.01).The TT,GT genotypes and T allele were more frequent,while GG genotype and G allele were less frequent,in the patients than in the controls.The GT genotype at position 817 was a risk factor for the production of LAC (P＜ 0.05,OR =2.33,95％CI =1.18-4.59),anti-β2GPl antibodies(P＜ 0.01,OR =5.92,95％CI =2.61-13.46) and ACA(P＜ 0.05,OR =2.52,95％CI =1.22-5.24),and the TT genotype was associated with an increased frequency of anti-β2GPI antibodies (P ＜ 0.01,OR =5.84,95％CI =1.69-20.20).Conclusions The 817G/T(Leu/Va1247) polymorphism in exon 7 of apoH gene is associated with the generation of APL antibodies in patients of Han nationality with SLE in Wenzhou region.The TT and GT genotypes at position 817 of apoH gene appear to be a risk factor for the production of APL antibodies.
