Expression and distribution of N-myc downstream regulated gene 2 in pancreatic diseases

VernacularTitle:胰腺疾病组织N-myc下游调节基因2的表达与分布
Author: Feifei HE ; Kai JIAO ; Xinping LIU ; Lan SHEN
Publication Type:Journal Article
Keywords: N-myc downstream regulated gene 2; Pancreatic islet cells; Insulin; Immunohistochemistry
From: Chinese Journal of Endocrinology and Metabolism 2013;(2):162-164
CountryChina
Language:Chinese
Abstract: The pancreatic tissues from patients with islet cell hyperplasia,insulinoma,and pancreatic adenocarcinoma,as well as normal pancreatic tissues were embedded with paraffin,serial sections were cut and mounted on glass slides.Immunohistochemical staining was carried out with N-myc down-stream regulated gene 2 (Ndrg2) monoclonal antibody by means of ABC method,and Western blotting was carried out to detect the expression and distribution of Ndrg2.The results showed that Ndrg2 positive immunoreactivity was mainly localized in the cytoplasm of islet cell,being similar to the localization of insulin positive immunoreactivity.The number and volume of pancreatic islets were increased in the patients with islet cell hyperplasia,and Ndrg2 expression was also increased.Western blotting results showed that the expression of Ndrg2 in the pancreas of patients with islet cell hyperplasia was increased compared with normal group.The above results suggest that Ndrg2 may play an important role in performing physiological function of islet cells.