Study of the association between a disintegrin and metalloprotease 19 gene polymorphisms and chronic obstructive pulmonary disease susceptibility
10.3760/cma.j.issn.0254-9026.2013.01.011
- VernacularTitle:去整合素基质金属蛋白酶19基因多态性与慢性阻塞性肺疾病易感性的研究
- Author:
Feng GUO
;
Limin DONG
;
Dongming LIU
;
Jiulong KUANG
- Publication Type:Journal Article
- Keywords:
Matrix metalloproteinases;
Polymorphism,restriction fragment length;
Pulmonary disease,chronic obstructive
- From:
Chinese Journal of Geriatrics
2013;(1):41-44
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the association between genetic polymorphism of a disintegrin and metalloprotease 19 (ADAM19) and chronic obstructive pulmonary disease (COPD).Methods Totally 116 patients with COPD (the COPD group) and 82 healthy volunteers (the control group) were enrolled in this study.Fasting peripheral blood was taken and whole blood corpuscle genomic DNA was extracted.The genetic polymorphism of ADAM19 (rs1422795) was determined by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP).Results The difference in genotypes frequencies (GG,AG,AA) distribution of the ADAM19 between patients (73.3％,22.4％,and 4.3％) and controls (68.3％,28.0％,and 3.7％) showed no statistically significance (x2 =0.887,P=0.634).There were also no significant differences in the distribution of the different allele gene frequencies(A,G) between patients (84.5％,15.5％) and controls(82.3％,17.7％)(x2=0.582,P=0.446).No differences were found in the distribution of the genotypes between patients of different COPD severity classified according to FEV1 (％ pred),and healthy controls(x2=3.325,P=0.787).Conclusions ADAM19 (rs1422795) genetic polymorphism is not related to the development of COPD,which may not be the COPD-related gene.
