Protective effect of portal vein occlusion without hepatic artery inflow control on cirrhotic rat liver
10.3760/cma.j.issn.1007-8118.2013.01.019
- VernacularTitle:保留肝动脉入肝血流阻断法对肝硬化大鼠肝脏的保护作用
- Author:
Jianjun HU
;
Chonghui LI
;
Hongdong WANG
;
Xu JI
;
Xinlan GE
;
Ke PAN
;
Jiahong DONG
- Publication Type:Journal Article
- Keywords:
Liver;
Blood inflow occlusion;
Reperfusion injury;
Liver cirrhosis;
Rats
- From:
Chinese Journal of Hepatobiliary Surgery
2013;(1):62-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to improve cirrhotic liver management,each aspect of the liver's complex blood flow must be understood.This study investigates the protective effect of portal vein occlusion,with hepatic artery preservation,on cirrhotic liver after ischemia and reperfusion.Methods Carbon tetrachlorideand induced cirrhotic rats and normal rats were randomly assigned into 4 groups:normal sham operation (N-SO),cirrotic sham operation (C-SO),portal triad clamping (PTC),and portal vein clamping without hepatic artery inflow control (PVC).During the occlusion,the total 3-minute blood loss from the liver surface cut was weighed.At 1,6,and 24 hours post reperfusion,the serum alapine amino transferas (ALT),the adenosine triphosphate (ATP) of liver tissue,the malonolialdehgde (MDA) of liver tissue,and the morphological changes were evaluated.Result The amount of hemorrhage between the groups ranked as follows:PTC ＜ PVC ＜ N-SO ＜ C-SO (P＜0.05).At 1,6,and 24 hours post reperfusion.the ALT and MDA levels of the groups ranked as follows:PTC ＞ PVC ＞ C-SO ＞ N-SO (P＜0.05).Additionally,each group's ATP level ranked as follows:PTC ＜ PVC ＜ C-SO ＜ N-SO (P＜0.05).With histopathological examination,the hepatic injuries of the PTC and PVC group were more severe than those of the C-SO group,especially in the PTC group.Conclusion Therefore,the technique of portal vein clamping and hepatic artery inflow control can reduce the ischemic reperfusion injury of the cirrhotic rats' liver.
