Relationship of 5-hydroxy indole acetic acid, melatonin and dopamine in rat model of Parkinson's disease induced by 6-hydroxydopamine
10.3760/cma.j.issn.1674-6554.2013.04.002
- VernacularTitle:6-羟基多巴胺诱导的帕金森病大鼠纹状体5-羟吲哚乙酸、褪黑素与多巴胺的关系
- Author:
Yuequn DU
;
Tao MENG
;
Zhihong ZHENG
;
Ling LIN
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
Dopamine;
5-hydroxy indole acetic acid;
Melatonin
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2013;(4):293-295
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate dynamic changes in extracellular striatal 5-hydroxy indole acetic acid (5-HIAA) and melatonin (MLT) concentrations in the 6-hydroxydopamine (6-OHDA)-induced Parkinson's disease (PD) rat models.Methods A hemiparkinsonium rat model was established by a unilateral injection of 6-OHDA into the right substantia nigra pars compacta (SNc) and the right medial forebrain bundle (MFB).Dialysate samples were collected at 10:00 in the lesioned striatum by microdialysis at 1,2,3 and 4 weeks post-lesion,respectively.The levels of dopamine (DA) and 5-HIAA,as well as MLT in dialysate sanples were measured by high performance liquid chromotorgraphy (HPLC).Results One week after surgery,the level of DA in PD group was (1.19±0.22) ng/ml,which was distinctly lower than those in the vehicle group ((8.62 ± 0.73) ng/ml) and the sham group ((8.73 ± 0.78) ng/ml) (P ＜ 0.01).The levels of 5-HIAA in PD group had a downward trend,but there were no significant differences.The level of MLT in PD group was (43.31 ± 11.37) pg/ml at 4 weeks post-lesion,and significantly higher than those in the vehicle group ((19.13 ± 5.38)pg/ml) and the sham group ((19.80 ± 6.96) pg/ml) (P＜ 0.05).The striatal dialysate DA and 5-HIAA concentrations had a good positive correlation in the PD group (r =0.867,P ＜ 0.01).Conclusion Decreased DA level concomitantly with changes of 5-HIAA and MLT in the ipsilateral striatum of 6-OHDA-induced PD rats,and these changes may be related to PD progression.
