Analysis of the phenominon of anticoagulant led to false platelet reducing and its the correcting method
10.3760/cma.j.issn.1008-6706.2013.03.033
- VernacularTitle:抗凝剂依赖假性血小板减少症检验结果分析及纠正方法
- Author:
Huiqiang LU
;
Xiao WU
;
Jianqiang ZHANG
;
Xiaoping LI
;
Zhongwei CHEN
;
Lei ZHOU
;
Dongmei TANG
- Publication Type:Journal Article
- Keywords:
Anticoagulants;
Platelet count;
Reality testing
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(3):396-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence and solution of pseudothrom bocytopenia (PTCP) caused by anticoagulant EDTA-K2.Methods Sysmex-2100 hematology analyzer was used to analyze PTCP-positive patients' blood samples,count the platelet in samples anticoagulant with EDTA-K2,sodium citrate and heparin,and used the manual counting methods.Results Platelets counts in anticoagulant bood by EDTA-K2 was significantly lower than anticoagulant bood by sodium citrate and heparin.Lots of aggregated platelets were appeared in the EDTA-K2 anticoagulant samples stained by Wright-Giemsa,while there was no aggregated platelets observed in sodium citrate anticoagulant samples and heparin anticoagulant samples.Conclusion Platelet aggregation in patients with PTCP causes patelet counts false reducation.The patients with PTCP is determined by measuring the whole blood (no anticoagulant)by automatic hematology analyzer immediately or manual couting method.
