Detecting AmpC β-lactamases from nosocomial Klebsiella pneumoniae infection and their resistance in ICU patients
10.3760/cma.j.issn.1008-6706.2013.06.001
- VernacularTitle:重症监护病房患者医院感染肺炎克雷伯菌产头孢菌素酶检测及耐药性分析
- Author:
Lijun WANG
;
Ping LIN
- Publication Type:Journal Article
- Keywords:
Nosocomial infection;
Klebsiella pneumoniae;
Cephalosporinase;
Resistance
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(6):801-803
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore cephalosporinase from nosocomial Klebsiella pneumoniae infection and their drug-resistant characteristics among the ICU patients so as to provide an instruction for rational clinical medication.Methods 67 samples were identified with VITEK-60 automated system.The strains producing AmpC β-lactamases were screened with cefoxitin disk diffusion method recommended by the CLSI,and confirmed by cefoxitin three-dimensional test.AmpC β-lactamase genotype was differentiated by PCR-sequencing and susceptibility tests which were done with the Kirby-Bauer method recommended by the CLSI.Results In the 67 isolates of Klebsiella pneumoniae,11 strains(16.4％) were found to produce AmpC β-lactamases.The drug-resistant genotype for all the 11 AmpC-positive strains was DHA-1.AmpC-producing strains developed multi-drug resistance,especially for broadspectrum β-lactam antibiotics and enzyme inhibitors.β-lactamases-producing strains were more resistant than non-producing ones.Conclusion Klebsiella pneumoniae in ICU patients has a higher proportion of producing AmpC β-lactamase strains,which are all of DHA-1 genotype and have multi-drug resistance.
