Correlation research between Gene Polymorphism of TNF-alpha Gene 863C_A and Thyroid Associated Ophtalmopathy in Zhuang Ethnic Population

VernacularTitle:肿瘤坏死因子-α及其基因863C_A多态性与壮族甲状腺相关眼病的相关性研究
Author: Wenhao MA ; Rui QIN
Publication Type:Journal Article
Keywords: Tumor necrosis factor; Gene polymorphism; Graves,disease
From: Chinese Journal of Primary Medicine and Pharmacy 2013;20(1):18-20
CountryChina
Language:Chinese
Abstract: Objective To explore correlation between gene polymorphism of TNF-alpha and thyroid associated ophtalmopa thy (TAO) in Zhuang ethnic population.Methods DNA and serum samples were collected in patients with TAO in Han/Zhuang population.ELISA was applied to detect level of TNF-alpha in serum and PCR was applied to detect polymorphism of TNF-alpha gene 863C_A.Results The levels of TNF-alpha in patients with TAO increased significantly compared with control group (P ＜ 0.05),and more significantly in Zhuang population compared with Han population (P ＜ 0.05) ;gene frequency of CA was significantly higher in patients with TAO in Zhuang population than that in Han population (P ＜ 0.05),while gene frequency of AA was significantly lower (x2 =0.770,2.145,all P ＜ 0.05).Conclusion Serum level of TNF-alpha increases during occurrence of TAO,and the differences of expression level between different nationalities are due to gene polymorphism of TNF-alpha.