Effects of microRNA-196a inhibitory sequences on HOXB8 expression in human pancreatic cancer PANC1 cells

VernacularTitle:MicroRNA-196a抑制序列对人胰腺癌PANC1细胞株HOXB8基因表达的影响
Author: Minghao LIU ; Yiqi DU ; Jianqiang LIU ; Jun GAO ; Hongyu WU ; Yanfang GONG ; Zhaoshen LI
Publication Type:Journal Article
Keywords: Pancreatic neoplasms; MicroRNA; MicroRNA-196a; HOXB8
From: Chinese Journal of Pancreatology 2013;(1):13-15
CountryChina
Language:Chinese
Abstract: Objective To investigate the effects of microRNA-196a(miR-196a) inhibitory sequences transfection on HOXB8 expression in PANC1 cells.Methods PANC1 cells were divided into control group,miR-196a inhibitory sequences group and siRNA control group.Liposomal transfection method was applied to transfect miR 196a inhibitory sequences and siRNA control into PANC1 cells.RT-PCR and Western blot were used to detect the expressions of miR-196a and HOXB8 mRNA and protein.Results After miR-196a inhibitory sequences transfection,when compared with that of siRNA control group,the expression of miR-196a was significantly decreased (0.05 ± 0.054 vs.0.839 ± 0.025,t =3.12,P ＜0.05) ; and the expression of HOXB8 mRNA was significantly increased by 1.57 folds (2.20 ± 0.07 vs.1.29 ± 0.10,t =3.86,P ＜ 0.05),the expression of HOXB8 protein was also obviously increased (0.90 ± 0.03 vs.0.40 ± 0.10,t =3.11,P ＜ 0.05).Conclusions MicroRNA-196a down-regulates the expression of HOXB8.