Determination of mangiferin in Baihe Zhimu decoction by HPCE
10.3760/cma.j.issn.1673-4246.2013.03.012
- VernacularTitle:高效毛细管电泳法测定芒果苷在百合知母汤中的含量
- Author:
Jinxia YANG
- Publication Type:Journal Article
- Keywords:
Baihe Zhimu decoction;
Mangiferin;
HPCE
- From:
International Journal of Traditional Chinese Medicine
2013;(3):231-233
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish HPCE method for determining the mangiferin in Baihe Zhimu decoction.Methods Rutin was used as internal standard.An uncoated fused silica capillary (77 cm×75 μm,effective length of 70 cm) was served as separation gate.The electrolyte buffer was composed of 12 mmol/L borax-MeOH (90 ∶ 10).20 kV voltages were applied and detection wavelength was set at 318nm.Results Mangiferin was successfully separated within 20 rin,the linear response range was 0.06～1.14 mg/ml.The average recovery was 99.0％.Conclusion The method was simple,rapid and well reproducible.It could be used as a reliable tool for the quantity control of Baihe Zhimu decoction and descriptions containing mangiferin.
